Dr. Yogesh Saini
Assistant Professor
Comparative Biomedical Sciences (CBS)

Biography:
Dr. Saini received his DVM (1996-2001) from College of Veterinary Sciences, Palampur (India) and MVSc in Veterinary Biochemistry and Biotechnology (2001-2003) from College of Veterinary Sciences, Pantnager (India). He completed his Senior Research Fellowship (2003-2005) at Indian Veterinary Research Institute, Izatnagar (India). He received his dual-PhD (Genetics and Toxicology) from Michigan State University in 2009. After completing his postdoctoral training (2010-2014) at University of North Carolina (UNC), he was appointed as a faculty member at Marsico Lung Institute. Dr. Saini Joined LSU in 2014 as an Assistant Professor.

Teaching Interests:
Respiratory Physiology (DVM Curriculum)
Macrophage Biology (Graduate Course)
Lunch and Science Seminar Series

Research Interests:
Pulmonary Immunology And Toxicology Laboratory

The focus of our research is to understand multiple aspects of lung biology and inflammatory lung diseases. Broadly, the research employs murine models of pathogens- and toxicant-induced inflammation. Using these models of lung inflammation, the research aims to understand the roles of macrophages in the orchestration of inflammatory responses. Unlike traditional view, emerging evidences suggest macrophage as a highly plastic cell type with likely roles in regulation of initiation, progression and resolution of inflammatory responses. Ongoing research in the laboratory has established several tools to characterize macrophage responses. Currently active projects include, understanding macrophage number maintenance in lung, studying macrophage activation heterogeneity, and understanding roles of macrophages in different murine models of lung inflammation. The ultimate goal of this research is to develop diagnostic biomarker of lung diseases and therapeutic interventions to modulate inflammatory responses. The laboratory uses wide range of in vitro and in vivo models and techniques including confocal microscopy, flow cytometry, clinical biochemistry, immunochemical staining, magnetic-associated cell sorting, cellular and proteomic analyses of biological samples, molecular biology (PCR, RT-PCR, Gene expression analyses etc.) and cytokine assays.

Experimental In Vivo Models
Cystic Fibrosis: The laboratory employs, Scnn1b-Tg mouse, a model of muco-obstructive lung disease that recapitulate human cystic fibrosis lung disease. We have crossed these mice to several other strains to introduce various defects in immune responses. We are focused on understanding how innate, innate lymphoid, and adaptive immune systems interact to shape mucobstructive lung disease in these mice.

Allergic Asthma: We are interested in understanding how myeloid cells, including macrophages and cDCs, orchestrate allergic asthma responses.

Ozone-induced Airway Disease: Our laboratory is investigating how ozone exposures modulate muco-obstructive airway diseases.

Second hand smoke (SHS)-induced COPD: We have recently published a manuscript highlighting the effect of early postnatal second hand smoke exposure on the development of muco-obstructive airways disease. Moving forward, we are interested in studying interactions between second hand smoke, airway bacterial clearance, and myeloid cell-mediated immune responses.

Lab News
Paper of the year
Student Awards
Article Highlight
SHS Study Published in JI
Invited Talk at Gordon Research Conference
Charles River Research Award
Invited Talk at Summer Research Seminar Series

Publications
Pubmed

Trainees
Graduate Students
Brandon Lewis
Thao Vo

Undergraduate Students
Allison Kidder
Hayden Tageant
Alex Walsh

Training Positions Available
Positions are available for motivated, research career-oriented undergraduate, graduate, and postdoctoral trainees. Interested candidates may email their CVs to ysaini@lsu.edu

Contact
E-mail: ysaini@lsu.edu
Phone: 225-578-9143
Office: 2421 VetMed
Lab: 2413, 2312, 2314 VetMed

 Awards & Honors:
  • 2019 Dean's Teacher Merit Honor Award (1st Year and 2nd Year)
  • 2019 LSU Alumni Association Rising Faculty Award
  • 2019 Zoetis Award for Veterinary Research Excellence
  • 2019 Outstanding New Environmental Scientist (ONES) Award
  • 2018 AAI Early Career Faculty Award
  • 2018 SOT Paper of the Year Award
  • 2018 Second Place Presentation Award (Brandon Lewis; PhD Candidate)
  • 2017 Comparative and Veterinary Student Award (Brandon Lewis; PhD Candidate)
  • 2017 Society of Toxicology Graduate Student Award (Brandon Lewis; PhD Candidate)
  • 2017 Toxicologic and Exploratory Pathology Charles River Award (Brandon Lewis; PhD Candidate)
  • 2017 Second Place Presentation Award (Brandon Lewis; PhD Candidate)
  • 2016 AAI Early Career Faculty Award
  • 2016 Gordon Conference (Invited Oral Presentation)
  • 2016 Second Place Presentation Award (Postdoctoral Fellow)
  • 2015 Best Poster Award (First Place)
  • 2012 Best Presentation Award (Second Place)
  • 2010 Roger O McClellan Research Award
  • 2009 Dissertation Completion Fellowship
  • 2009 New Investigator Award (ASIO/SOT)
  • 2009 Young Investigator Award (New York Academy of Sciences)
  • 2008 Phi Zeta Presentation Award (First Place)
  • 2008 Best Absract Award (Metals Specialty Section- SOT)
  • 2003 CSIR Senior Research Fellowship
  • 2001 ICAR Junior Research Fellowship
  • 1996 Merit Scholarship (Highest Rank)
 Education:
  • Post doctoral fellowship, University of North Carolina
  • Dual-PhD, Genetics and Integrative Toxicology, Michigan State University
  • MVSc, College of Veterinary and Animal Sciences, GB Pant University of Agriculture and Technology, India
  • DVM, College of Veterinary and Animal Sciences, CSK HP Agricultural University, India
 Grants:
  • Understanding Myeloid Signaling in Ozone Induced Lung Diseases (2019-2024), R01, $2,672,000.00
  • The roles of myeloid cells in allergic inflammation (2019-2022), P20: COBRE, $825,000.00
  • Targeting cell‐specific responses to treat lung infections , Louisiana Board of Regents , $199,000.00
  • Myeloid hypoxia signaling in muco-obstructive lung diseases (2018-2019), COBRE pilot project, $50,000.00
  • Young Clinical Scientist Independent Research Grant on "The role of macrophages in lung inflammation", FAMRI, $488,250.00
  • The role of Hypoxia signaling in bacterial clearance (2016-2017), COBRE pilot project, $50,000.00
  • "Identification of macrophage - and epithelium-derived inflammatory signatures in the airways of murine model of chronic obstructive pulmonary disease (COPD)", LSU Veterinary School - USDA CORP grant, $10,000.00
 Publications:
Journal Article, Academic Journal:
  • Effect of LysM+ macrophage depletion on lung pathology in mice with chronic bronchitis., Saini, Yogesh; Lewis, B; Yu, D; Dang, H; Livraghi-Butrico, A; Del Piero, Fabio; O'Neal, W; Boucher, R, 2018, Physiological reports , Volume: 6, Number: 8, Pages: e13677
  • Loss of β Epithelial Sodium Channel Function in Meibomian Glands Produces Pseudohypoaldosteronism 1-Like Ocular Disease in Mice., Saini, Yogesh; Yu, D; Chen, G; Dang, A.; Burns, K; Wang, Y; Davis, R; Randell, S; Esther, Jr., C.; Paulsen, F; Boucher , R, 2018, The American Journal of Pathology, 188(1), 95-110
  • Early Postnatal Secondhand Smoke Exposure Disrupts Bacterial Clearance and Abolishes Immune Responses in Muco-Obstructive Lung Disease, Lewis, B; Sultana, R; Sharma, R; Noel, Alexandra; Langohr, Ingeborg; Patial, Sonika; Penn, Arthur; Saini, Yogesh, 2017, Journal of Immunology , Volume: 199, Number: 3, Pages: 1170-1183
  • Neonatal Pulmonary Macrophage Depletion Coupled to Defective Mucus Clearance Increases Susceptibility to Pneumonia and Alters Pulmonary Immune Responses., Saini, Yogesh; Wilkinson, K; Terrell, K; Burns, K; Livraghi-Butrico, A; Doerschuk, C; O'Neal, W; Boucher, R, 2016, American journal of respiratory cell and molecular biology , Volume: 54, Number: 2, Pages: 210-21
  • AngiotensinII-induced hypertensive renal inflammation is mediated through HMGB1-TLR4 signaling in rat tubuloepithelial cells, Nair, Anand; Ebenezer, Philip; Saini, Yogesh; Francis, Joseph, 2015, Experimental Cell Research , Volume: 335, Number: 2, Pages: 238-47
  • Loss of Hif-2α Rescues the Hif-1α Deletion Phenotype of Neonatal Respiratory Distress In Mice., Saini, Yogesh; Proper, S; Dornbos, P; Greenwood, K; Kopec, A; Lynn, S; Grier, E; Burgoon, L; Zacharewski, T; Thomas, R; Harkema, J; LaPres, J, 2015, PloS one , Volume: 10, Number: 9, Pages: e0139270
  • Hypoxia Inducible Factors Modulate Mitochondrial Oxygen Consumption and Transcriptional Regulation of Nuclear-Encoded Electron Transport Chain Genes, Saini, Yogesh; Hyejin, Hwang; Lynn, Scott; Vengellur, Ajith; Grier, E; Fergusan-Miller, S; LaPres, John, 2015, Biochemistry , Volume: 54, Number: 24, Pages: 3739-3748
  • Gene expression in whole lung and pulmonary macrophages reflects the dynamic pathology associated with airway surface dehydration., Saini, Yogesh, 2014, BMC Geonomics
  • Loss of hypoxia-inducible factor 2 alpha in the lung alveolar epithelium of mice leads to enhanced eosinophilic inflammation in cobalt-induced lung injury., Proper, S; Saini, Yogesh; Greenwood, K; Bramble, L; Downing, N; Harkema, J; Lapres, J, 2014, Toxicological sciences : an official journal of the Society of Toxicology , Volume: 137, Number: 2, Pages: 447-57
  • Neonatal epithelial hypoxia inducible factor-1α expression regulates the response of the lung to experimental asthma., Greenwood, K; Proper, S; Saini, Yogesh; Bramble, L; Jackson-Humbles, D; Wagner, J; Harkema, J; LaPres, J, 2012, American journal of physiology. Lung cellular and molecular physiology , Volume: 302, Number: 5, Pages: L455-62
  • The role of hypoxia-inducible factor-1α in acetaminophen hepatotoxicity., Sparkenbaugh, E; Saini, Yogesh; Greenwood, K; LaPres, J; Luyendyk, J; Copple, B; Maddox, J; Ganey, P; Roth, R, 2011, The Journal of pharmacology and experimental therapeutics , Volume: 338, Number: 2, Pages: 492-502
  • G-protein coupled receptor kinase 5 mediates lipopolysaccharide-induced NFκB activation in primary macrophages and modulates inflammation in vivo in mice., Patial, Sonika; Shahi, S; Saini, Yogesh; Lee, T; Packiriswamy, N; Appledorn, D; Lapres, J; Amalfitano, A; Parameswaran, N, 2011, Journal of cellular physiology , Volume: 226, Number: 5, Pages: 1323-33
  • Myeloid-specific GPCR kinase-2 negatively regulates NF-κB1p105-ERK pathway and limits endotoxemic shock in mice., Patial, Sonika; Saini, Yogesh; Parvataneni, S; Appledorn, D; Dorn, 2nd, G; Lapres, J; Amalfitano, A; Senagore, P; Parameswaran, N, 2011, Journal of cellular physiology , Volume: 226, Number: 3, Pages: 627-37
  • Acute cobalt-induced lung injury and the role of hypoxia-inducible factor 1alpha in modulating inflammation., Saini, Yogesh; Greenwood, K; Merrill, C; Kim, K; Patial, Sonika; Parameswaran, N; Harkema, J; LaPres, J, 2010, Toxicological sciences : an official journal of the Society of Toxicology , Volume: 116, Number: 2, Pages: 673-81
  • Role of hypoxia-inducible factor 1{alpha} in modulating cobalt-induced lung inflammation., Saini, Yogesh; Kim, K; Lewandowski, R; Bramble, L; Harkema, J; Lapres, J, 2010, American journal of physiology. Lung cellular and molecular physiology , Volume: 298, Number: 2, Pages: L139-47
Scientific Abstract:
  • Understanding macrophage recruitment to the stressed airspaces, Sultana, R; Lewis, B; Mohammad, Owais; Saini, Yogesh, 2016
  • In Vitro Screening Method For Characterization Of Macrophage Activation Responses To Environmental Stress., Lewis, B; Sultana, R; Saini, Yogesh, 2016
  • Blockage of the P2y14-receptor inhibits neutrophil infiltration in chronic lung diseases, Sesma, J; Livraghi-Butrico, A; Wilkinson, K; Weitzer, C; Saini, Yogesh, 2015