Dr. Vladimir Chouljenko
Pathobiological Sciences (PBS)

Research Interests:
My main research interests are concentrated on the molecular biology and pathogenesis of human herpes simplex virus (HSV-1). Our experimental approach utilizes advanced molecular biology, molecular genetics and cell biology techniques. Specifically, mutant herpesviruses deficient in a particular function are isolated through site-specific mutagenesis of viral genomes cloned into bacterial artificial chromosomes (BAC).
A second aspect of my research is the molecular biology and pathogenesis of human and bovine coronaviruses. The entire genomic nucleotide sequence of several respiratory and enteric bovine coronaviruses has been produced and specific nucleotide changes, which may be associated with virus tropism has been identified.
A third part of my research involves next generation sequencing technology (NGS) using Illumina sequencing platform for rapid DNA sequencing of different viral and bacterial genomes as well as both a single cell and whole genome transcriptome RNA sequencing.

 Grants:
  • Center for Pre-Clinical Disease Research , National Institutes of Health - NIH, $11,192,691.00
 Publications:
Journal Article, Academic Journal:
  • Intramuscular vaccination of mice with the human herpes simplex virus type-1(HSV-1) VC2 vaccine, but not its parental strain HSV-1(F) confers full protection against lethal ocular HSV-1 (McKrae) pathogenesis., Naidu, S; Nabi, R; Cheemarla, N; Stanfield, Brent; Rider, Paul; Jambunathan, N; Chouljenko, Vladimir; Carter, Renee; Del Piero, Fabio; Langohr, Ingeborg; Kousoulas, Konstantin, 2020, PloS one , Volume: 15, Number: 2, Pages: e0228252
  • Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus Type-1(HSV-1) Vaccine Strain VC2 Expressing the Equine Herpesvirus-1 (EHV-1) Glycoprotein D (gD) Generates Anti-EHV-1 Immune Responses in Mice, Liu, Shiliang; Stanfield, Brent; Chouljenko, Vladimir; Naidu, Shan; Langohr, Ingeborg; Del Piero, Fabio; Ferracone, Jacqueline; Roy, Alma; Kousoulas, Konstantin, 2017, Journal of Virology , Pages: JVI.02445-16
  • Vaccination of rhesus macaques with the live-attenuated HSV-1 vaccine VC2 stimulates the proliferation of mucosal T cells and germinal center responses resulting in sustained production of highly neutralizing antibodies., Stanfield, Brent; Pahar, B; Chouljenko, Vladimir; Veazey, R; Kousoulas, Konstantin, 2017, Vaccine , Volume: 35, Number: 4, Pages: 536-543
  • Draft Genome Sequences of Edwardsiella ictaluri Strains LADL11-100 and LADL11-194 Isolated from Zebrafish Danio rerio., Wang, R; Tekedar, H; Lawrence, M; Chouljenko, Vladimir; Kim, J; Kim, N; Kousoulas, Konstantin; Hawke, John, 2015, Genome announcements , Volume: 3, Number: 6
  • Deletion of a Predicted β-Sheet Domain within the Amino Terminus of Herpes Simplex Virus Glycoprotein K Conserved among Alphaherpesviruses Prevents Virus Entry into Neuronal Axons., Jambunathan, N; Charles, A; Subramanian, R; Saied, A; Naderi, M; Rider, P; Brylinski, M; Chouljenko, Vladimir; Kousoulas, Konstantin, 2015, Journal of virology , Volume: 90, Number: 5, Pages: 2230-9
  • Genomic diversification in strains of Rickettsia felis Isolated from different arthropods., Gillespie, J; Driscoll, T; Verhoeve, V; Utsuki, T; Husseneder, C; Chouljenko, Vladimir; Azad, A; Macaluso, Kevin, 2014, Genome biology and evolution , Volume: 7, Number: 1, Pages: 35-56
  • A single intramuscular vaccination of mice with the HSV-1 VC2 virus with mutations in the glycoprotein K and the membrane protein UL20 confers full protection against lethal intravaginal challenge with virulent HSV-1 and HSV-2 strains., Stanfield, Brent; Stahl, J; Chouljenko, Vladimir; Subramanian, Ramesh; Charles, A; Saied, A; Walker, J; Kousoulas, Konstantin, 2014, PloS one , Volume: 9, Number: 10, Pages: e109890