Dr. Vladimir Chouljenko
Pathobiological Sciences (PBS)
My main research interests are concentrated on the molecular biology and pathogenesis of human herpes simplex virus (HSV-1). Our experimental approach utilizes advanced molecular biology, molecular genetics and cell biology techniques. Specifically, mutant herpesviruses deficient in a particular function are isolated through site-specific mutagenesis of viral genomes cloned into bacterial artificial chromosomes (BAC).
A second aspect of my research is the molecular biology and pathogenesis of human and bovine coronaviruses. The entire genomic nucleotide sequence of several respiratory and enteric bovine coronaviruses has been produced and specific nucleotide changes, which may be associated with virus tropism has been identified.
A third part of my research involves next generation sequencing technology (NGS) using Illumina sequencing platform for rapid DNA sequencing of different viral and bacterial genomes as well as both a single cell and whole genome transcriptome RNA sequencing.