Dr. Masami Yoshimura
Associate Professor
Comparative Biomedical Sciences (CBS)

Research Interests:
Cyclic AMP (cAMP), a ubiquitous second messenger in eukaryotic cells, plays a regulatory role in diverse biological processes including sugar and lipid metabolism, cell growth and differentiation, cardiac contractility, olfaction, as well as learning and memory. cAMP generation is controlled by a variety of hormones, neurotransmitters, and other extracellular molecules. This second messenger directly activates diverse molecules including protein kinase A, cyclic nucleotide-gated channels, and Epac, a guanine nucleotide exchange factor for the Ras-like small GTPase Rap1 and Rap2. The common form of signal transduction system that generates cAMP is a membrane-bound multi-component system. This system consists of various G protein-coupled receptors (GPCRs) which recognize extracellular signals, heterotrimeric G proteins, and membrane-bound adenylyl cyclase (AC), the enzyme that converts ATP to cAMP. Synthesis of cAMP by AC is a major determinant of the intracellular concentration of cAMP. Mammals, including humans, have nine different membrane-bound AC isoforms, type 1 to type 9 (AC1-AC9), and one soluble AC isoform (sAC). Each AC isoform has a distinct regulatory profile and tissue distribution. The activity of each isoform is controlled quite differently by a variety of factors. Regulation of a given AC isoform by multiple stimuli allows that isoform to function as a specific integrator of the external stimuli and further allows that isoform to play an interpretive role in signal transduction. AC isoforms could be important targets for a new generation of tissue- and cell-specific drugs or therapeutic interventions against various health problems.
I have been interested in how each isoform of AC is regulated by endogenous and exogenous factors. I started my research in the field of cAMP signal transduction by cloning mammalian AC cDNAs and genes. Our research found that drinking alcohol (ethanol) enhances the activity of AC in an isoform-specific manner and that AC7 is most ethanol responsive. We have studied the mechanisms responsible for this isoform-specific regulation. Now we shift our research focus to study the role of AC7 in the effect of alcohol drinking on the pathophysiology of live animals. Alcohol use increases the incidence of pneumonias and other infections caused by bacteria and viruses, and can negatively affect recovery from infections and traumatic injuries. Because of AC7’s high ethanol responsiveness and its high expression in immune cells, we hypothesize that AC7 is a primary factor that controls ethanol’s effects on immune responses. Currently, we study innate immune responses of the lung as a model to determine the role of AC7 in the effects of alcohol drinking. We use genetically modified mice and cell lines as model systems. Research methods employed include qRT-PCR, ELIZA, FRET analysis, RNAScope, flow cytometry, and functional analyses of macrophages such as phagocytosis, chemotaxis, nitric oxide generation, and bactericidal activity.

 Awards & Honors:
  • 2021 Dean's Teacher Merit Honor Roll
  • 2020 Dean's Teacher Merit Honor Roll
  • 2019 Dean's Teacher Merit Honor Roll
  • 2018 Dean's Teacher Merit Honor Roll
  • 2017 Dean's Teacher Merit Honor Roll
  • 2016 Dean's Teacher Merit Honor Roll
  • 2015 Dean's Teacher Merit Honor Roll
  • 2014 Dean's Teacher Merit Honor Roll
  • 2013 Dean's Teacher Merit Honor Roll
  • 2011 Dean's Teacher Merit Honor Roll
  • 2010 Pfizer Award for Research Excellence
 Grants:
  • Suppression of lung innate immune responses by acute alcohol exposure: role of adenylyl cyclase type 7., LSU School of Veterinary Medicine,
  • Effects of alcohol consumption on the expression of adenylyl cyclase type 7, CBS/SVM/LSU, $5,000.00
  • The role of AC7 in innate immune responses in acute lung injury induced by LPS and alcohol , CENTER FOR LUNG BIOLOGY AND DISEASE (COBRE), $30,000.00
  • The role of adenylyl cyclase type 7 in alcohol’s effects on immune responses, Endotoxemia in AC7 conditional knockout mice as a model system., CBS/SVM/LSU, $5,000.00
  • Generation and innate immune study of Type 7 Adenylyl Cyclase knock-out cell lines from human myeloid cell line HL-60, CBS/SVM/LSU, $5,000.00
  • Role of type 7 adenylyl cyclase in Innate Immunity, LSU Biomedical Collaborative Research Program, $50,000.00
  • Role of Type 7 Adenylyl Cyclase in Ethanol’s Effects on Immune Response, LSU School of Veterinary Medicine, $9,000.00
  • The role of cAMP signaling in alcohol's effect on the immune response, CBS/SVM/LSU, $10,000.00
  • Generation of gene knockout cell line from BV-2 microglia cell line, LSU School of Veterinary Medicine, $10,000.00
  • Studies of Effects of Adenylyl Cyclase 7 on CREB Activation and Its regulated Gene Expression Upon Acute Ethanol Stimulation, Louisiana Biomedical Research Network,
 Publications:
Scientific Abstract:
  • EFFECT OF ALCOHOL ON PULMONARY IMMUNE RESPONSES OF MYELOID LINEAGE-SPECIFIC AC7 KO MICE, Hu, Yawen; Yoshimura, Masami, 2020
  • Alcohol effects on immune responses of classical pro-inflammatory macrophages , Hu, Yawen; Yoshimura, Masami, 2019
  • Effects of Alcohol on Innate Immune Responses of BV-2 Microglial Cell Line without Type 7 Adenylyl Cyclase, Hu, Yawen; Yoshimura, Masami, 2018
  • Role of AC7 in Alcohol’s Effect on Cyclic AMP Regulated Reporter Gene Expression in RAW 264.7 and BV-2 Immune Cells, Hill, R; wu, X; Yoshimura, Masami, 2016
  • Effects of Alcohol on Expression of a Cyclic AMP Regulated Gene in Mammalian Cells, Hill, Rebecca; Xu, Wu; Yoshimura, Masami, 2015
  • Effects of Alcohol on Expression of Cyclic AMP Regulated Gene in Mammalian Cells, Hill, Rebecca; Xu, Wu; Yoshimura, Masami, 2014
  • Effects of Alcohol on Expression of Cyclic AMP Regulated Gene in Mammalian Cells, Hill, Rebecca; Xu, Wu; Yoshimura, Masami, 2013
  • Effects of alcohol on expression of cyclic AMP regulated gene in mammalian cells, Hill, Rebecca; Qualls-Creekmore, Emily; Xu, Wu; Yoshimura, Masami, 2013
Journal Article, Academic Journal:
  • The effect of alcohol on recombinant proteins derived from mammalian adenylyl cyclase , Qualls-Creekmore, E; Gupta, R; Yoshimura, Masami, 2017, Biochemistry and Biophysics Reports , Volume: 10, Pages: 157-164
  • Observation on the ultrastructure morphology of HeLa cells treated with ethanol: Statistical analysis., Al-Bagdadi, Fakhri; Matthew, J; Young, J; Geaghan, J; Yao, Shaomian; Barona, H; Martinez-Ceballos, E; Yoshimura, Masami, 2016, Ultrastructural Pathology , Volume: 15, Number: 30
  • Observation on the ultrastructure morphology of HeLa cells treated with ethanol: Statistical analysis, Al-Bagdadi, Fakhri; Young, PhD , Matthew ; Geaghan, PhD, James ; Yao, Shaomian; Barona, PhD , Humberto; Martinez-Ceballos, PhD, Eduardo; Yoshimura, Masami, 2016, J Microsc Ultrastruct , Volume: 4, Number: 1, Pages: 46-54
  • Role of an adenylyl cyclase isoform in alcohol’s effect on cAMP regulated gene expression in NIH 3T3 cells, Hill, Rebecca; Xu, Wu; Yoshimura, Masami, 2016, Biochemistry and Biophysics Reports , Volume: 8, Pages: 162-167
  • Real-time monitoring of intracellular cAMP during acute ethanol exposure, Gupta, Ratna; Qualls-Creekmore, Emily; Yoshimura, Masami, 2013, Alcohol.Clin.Exp.Res. , Volume: 37, Pages: 1456-1465
  • Effects of Alcohols on Recombinant Adenylyl Cyclase Type 7 Expressed in Bacteria, Dokphrom, Usa; Qualls-Creekmore, Emily; Yoshimura, Masami, 2011, Alcoholism: Clinical and Experimental Research , Volume: 35, Number: 11, Pages: 1915-1922
  • Neurotransplatation of genetically modified stem cells alters thanol preference., Grammatopoulos, T; Jones, S; Yoshimura, Masami; Hoover, B; Doolen, S; Snyder, E; Zahniser, N; Tabakoff, B; Zawada, W, 2010, Stem Cell Research & Therapy , Volume: 1, Pages: 36 (13 pages)
  • Effects of straight chain alcohols on specific isoforms of adenylyl cyclase., Hasanuzzaman, M; Yoshimura, Masami, 2010, Alcohol. Clin. Exp. Res. , Number: 34, Pages: 743-749